Journal: Molecular & Cellular Proteomics : MCP

Article Title: Omics and Multiomics-Based Diagnostics for Invasive Candidiasis: Toward Precision Medicine

doi: 10.1016/j.mcpro.2025.101463

Figure Lengend Snippet: Overview of the different omics approaches currently applied to IC diagnosis. Methods approved by the FDA for IC diagnosis are indicated. Asterisks show technologies that have been used for the detection of Candida colonization or infection forms other than IC. See text for further details. 1 H-NMR, proton nuclear magnetic resonance; Cas, CRISPR-associated protein; CRISPR, clustered regularly interspaced short palindromic repeats; ESI, electrospray ionization; FDA, Food and Drug Administration; FT-IR, Fourier transform infrared; GC, gas chromatography; GWAS, genome-wide association study; HILC, hydrophilic interaction liquid chromatography; IAMS, immunoaffinity mass spectrometry; IC, invasive candidiasis; IVIAT, in vivo induced antigen technology; LC, liquid chromatography; MALDI-TOF, matrix-assisted laser desorption/ionization time-of-flight; mNGS, metagenomic NGS; metaRNA-seq, meta-analysis of RNA-seq; MRI, magnetic resonance imaging; MRM, multiple reaction monitoring; MS, mass spectrometry; NGS, next-generation sequencing; NPS, nanopore sequencing; NPmGS, nanopore-based metagenomic sequencing; PCR, polymerase chain reaction; PET, positron emission tomography; PNA-FISH, peptide nucleic acid-fluorescent in situ hybridization; REIMS, rapid evaporative ionization mass spectrometry; RNA-seq, RNA sequencing; SEREX, serological analysis of recombinant complementary DNA expression libraries; SERPA, serological proteome analysis; SERS, surface enhanced Raman scattering; SPR, surface plasmon resonance; SRS, single-cell stimulated Raman scattering; T2MR, T2 magnetic resonance; TGS, third-generation sequencing; UPLC, ultra-performance liquid chromatography; WGS, whole genome sequencing; WmGS, whole metagenome sequencing; xMAP, multi-analyte profiling (x, analyte).

Article Snippet: However, the commercially available PCR-surface-enhanced Raman scattering assay (the RenDx Fungiplex assay (Renishaw Diagnostics Ltd)) lacks clinical evaluation in large patient cohorts ( ).

Techniques: Biomarker Discovery, Infection, Nuclear Magnetic Resonance, CRISPR, Fourier Transform Infrared Spectroscopy, Gas Chromatography, GWAS, Hydrophilic Interaction Liquid Chromatography, Mass Spectrometry, In Vivo, Liquid Chromatography, RNA Sequencing, Magnetic Resonance Imaging, Targeted Proteomics, Next-Generation Sequencing, Nanopore Sequencing, Sequencing, Polymerase Chain Reaction, Positron Emission Tomography, In Situ Hybridization, Recombinant, Expressing, SPR Assay